Background & Objectives: The researchers aim to determine the antifungal activity of the fixed oil extracted from the pili nut peel waste (pulp, shell, and seed coat) using the Resazurin Microbial Assay. The study also aims to determine the yield, physiocochemical properties and the active constituents present in the fixed oil obtained from the Pili nut peel wastes.
Methods: The fresh ripe Pili nut peel wastes were collected and cut and ground into small pieces for extraction using the Soxhlet apparatus. After the fixed oil was obtained, it is tested into different physio-chemical tests, undergone phytochemical screening, and subjected into biological assay, the resazurin microbial assay, which is a fluorometric determination of the antifungal activity againts the common fungal pathogen (C. albicans, A. niger, and T. rubrum).
Results: The antifungal activity was determine through 10ug/mL, 50ug/mL, 100ug/mL and 1000ug/mL concentrations for the assay and it was compared to positive control and negative control with the same concentrations. The results were interpreted using ANOVA, T-Test, and Tukey's multiple comparison method.
Conclusion: The fixed oil showed higher inhibitory activity against systemic fungal pathogens. The percentage inhibition produced by the fixed oil was comparable to that of the reference standard, fluconazole. However, its activity against Trichophyton rubrum, a dermotophyte, was significantly lower compared to the reference standard, ketoconazole. The results suggest higher efficacy of the fixed oil against systemic fungal pathogens and lower efficacy of the fixed oil against systemic fungal pathogens and lower efficacy against local fungal pathogens such as dermatophytes.